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KMID : 0364819880260010032
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Korean Journal of Microbiology
1988 Volume.26 No. 1 p.32 ~ p.36
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Molecular Cloning and Restriction Analysis of Aspartokinase Gene (HOM3) in the Yeast, Saccharomyces cervisiae
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Choi Seoung-Il
Lea Ho-Zoo
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Abstract
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1
The yeast gene HOM3 encodes aspartokinase, which catalyses the first step (aspartate to and from beta-aspartyl phosphate) of common pathway to threonine and methionine. The yeast HOM3 gene expression is known to be regulated by threonine and methionine specific control, and also by general control of amino add biosynthesis. Isolation and characterization of the HOM3 gene are essential for the molecular genetic study on its regulation of expression. A recombinant plasmid pSC3 (15.5kb, vector YCp5O) has been cloned into E. coil HB191 from yeast genomic library through their complementing activity of HOM3 mutation in a yeast recipient strain M34-24B. Organization of the plasmid was characterized by delineation of restriction cleavage sites in the insert fragment.
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